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Image Search Results
Journal: Journal of Korean Medical Science
Article Title: Emergence and Wide Dissemination of CTX-M-type ESBLs, and CMY-2- and DHA-1-type AmpC β-Lactamases in Korean Respiratory Isolates of Klebsiella pneumoniae
doi: 10.3346/jkms.2005.20.6.961
Figure Lengend Snippet: PFGE band pattern of 74 strains of K. pneumoniae were analyzed with UVIBand Map V.99 by the un-weighted pair group method using arithmetic averages (UPGMA). * The β-lactamases produced include S12+C2 (18), S12+D1 (1), and CT14+C2 (2). C1, CMY-1; C2, CMY-2; CT14, CTX-M-14; D1, DHA-1; S12, SHV-12; T52, TEM-52.
Article Snippet: The pulsed-field gel electrophoresis (PFGE) band patterns were analyzed with the
Techniques: Produced
Journal: Scientific reports
Article Title: Uterine extracellular vesicles as multi-signal messengers during maternal recognition of pregnancy in the mare.
doi: 10.1038/s41598-022-19958-z
Figure Lengend Snippet: Figure 1. Characterization of uterine extracellular vesicles (uEVs). (A) Representative images (transmission electron microscopy) of EVs samples isolated from uterine lavages from pregnant mares on days 10 (P10), 11 (P11), 12 (P12), and 13 (P13), and from cyclic mares (controls) on days 10 (C10) and 13 (C13) after final ultracentrifugation and referred as uEVs. (B) Western blot characterization of uEVs (100,000 g) and the 12,000 g pellet for known exosomal protein markers CD9, TSG101, ALIX (PDCD6IP) and HSP70. (C) Comparison of uEVs concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300). (D) Comparison of uEVs protein concentration across samples measured by Pierce™ BCA protein assay. (E) Comparison of uEVs RNA concentration across samples measured by Quantus™ Fluorometer.
Article Snippet: Antibodies and dilutions used for Western Blotting experiments were as follow: For primary antibodies, CD9 antibody (Mouse anti Human CD9, clone MM2/57, MCA469GT, specified as cross-reactive with equine CD9 by the provider Bio-Rad), dilution 1:500; TSG101 Polyclonal Antibody (PA5-31,260, rabbit, specificity demonstrated by shRNA mediated knockdown of the target protein, Invitrogen), 1:1000; Anti-ALIX antibody (1A12, mouse, monoclonal, sc-53540, Santa Cruz Biotechnology, Inc., Heidelberg, Germany, specificity shown in88), 1:500;
Techniques: Transmission Assay, Electron Microscopy, Isolation, Western Blot, Comparison, Concentration Assay, Protein Concentration, Bicinchoninic Acid Protein Assay